SRT1720

SRT1720 enhances maturity and quality of oocytes in aged mice

Mei-Ju Liu1,2 | Hong Chen1,3 | Wen Li1 | Xin-Xia Chen4 | Chuan-Hong Wang1,3 | Qing-Yuan Sun5 | Xue-Yuan Heng6

Abstract
This study aims to investigate the morphology and distribution of mitochondria, spin- dles, and chromosomes in oocytes of aged mice and examine the effects of SRT1720 on oocyte maturation. C57BL/6J mice were divided into young (4–8 weeks) and aged groups (48–52 weeks). In vitro maturation media contained (0.05, 0.1, and
1.0 μM) SRT1720 and 0.1-μM dimethyl sulfoxide (DMSO control). The rate of chro- mosome misalignment and spindle misorientation in oocytes of aged mice were sig- nificantly higher than that of young mice (P < 0.01). Fluorescence intensity of mitochondria from oocytes of aged mice was significantly lower than that of young mice (P < 0.01). SRT1720 at 0.1 μM significantly improved oocyte maturation, fertili- zation, and blastocyst formation in aged mice compared with young mice (P < 0.01). Additionally, immunofluorescence intensity of mitochondria, normal spindle morphol- ogy, and chromosome alignment were notably enhanced with SRT1720 when com- pared with the DSMO control group for metaphase II (MII)-stage oocytes matured in vitro (P < 0.01); 0.1-μM SRT1720 enhanced the expression level of SRIT1 in oocytes from aged mice. In summary, the aged mice oocytes showed increased nuclear and cytoplasmic defects, whereas SRT1720 enhanced oocyte maturation and quality. We concluded that 0.1-μM SRT1720 was an appropriate concentration for in vitro maturation media.

KE YW OR DS
aged mice, IVM, oocytes, organelles cytoskeleton, SRT1720
1Department of Reproductive Medicine, Linyi People’s Hospital, Shandong University, Linyi, China
2Institute of Zoology, Chinese Academy of Sciences, Beijing, China
3School of Clinical Medicine, Shandong First Medical University, Tai’an, China
4School of Nursing, Shandong University, Jinan, China
5Fertility Preservation Lab, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, China
6Department of Neurological Surgery, Linyi People’s Hospital, Shandong University, Linyi, China

Correspondence
Qing-Yuan Sun, Fertility Preservation Lab, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou 510317, China.
Email: [email protected]
Xue-Yuan Heng, Department of Neurological Surgery, Linyi People’s Hospital, Shandong University, Jiefang Road, Linyi, Shandong 276000, China.
Email: [email protected]

Funding information
Medical Doctor Innovation Fund of Linyi People’s Hospital, Grant/Award Number: 2019LYBS07; Joint Research Funding of Shandong University and Karolinska Institute, Grant/Award Number: SDU-KI-2019-08; Shandong Provincial Medicine and Health Technology Development Project, Grant/ Award Number: 2018WS338

1 | INTRODUCTION

Evidence has suggested that the fertility rate of women decreases with age (Speroff, 1994). The reason that advanced maternal age

Qing-Yuan Sun and Xue-Yuan Heng contributed equally.

causes decreased fertility includes decreasing ovarian reserve and poor oocyte quality, leading to a fall in pregnancy rates (Yan et al., 2012). Mitochondria are crucial organelles, not only for provid- ing energy but also for regulating calcium homeostasis and apoptosis in human oocytes (Perez et al., 2000). Besides mitochondria, correct meiotic spindle integrity and chromosome arrangement play

Anim Sci J. 2021;92:e13608. wileyonlinelibrary.com/journal/asj © 2021 Japanese Society of Animal Science https://doi.org/10.1111/asj.13608
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important roles in ensuring normal fertilization and embryo develop- ment. Evidence has suggested that there is deficient spindle forma- tion, misaligned chromosomes, and abnormal mitochondria during oocyte maturation in aged mice (Jian et al., 2014).
About 15% to 20% of collected oocytes are immature when using external gonadotropin hormone for oocyte stimulation (Reichman et al., 2010). Therefore, immature oocytes may provide an opportu- nity for patients who are lacking oocytes because of advanced age. However, some authors have pointed out that oocytes from in vitro maturation culture might increase the rate of meiotic abnormalities and chromosome misalignment (Vieira et al., 2011). Therefore, we decided to explore ways in which maturation and oocyte quality could be improved.
SRT1720 is a new chemical entity drug produced by the GlaxoSmithKline subsidiary. SRT1720 has strong antiaging and anti- apoptosis properties when used in vitro (Li et al., 2016) and improves mitochondria respiratory chain function by activating the SIRT1 path- way. SIRT1 is part of a family of proteins called sirtuins, which are class III histone deacetylases (Armstrong & Que, 2012). Recent studies have described how SIRT1 play a crucial role in mitochondrial biogen- esis and autophagy. An emerging important role of SIRT1 in protecting oocytes from maternal aging has also been reported (Gomes et al., 2013; Tatone et al., 2018). Resveratrol, a natural SIRT1 activator, prolongs ovarian lifespan and protects against age- associated infertility in mammals (Chen et al., 2010; Liu et al., 2013). However, resveratrol not only activates SIRT1 but also activates other signaling pathways (Beher et al., 2009). Although evidence has emerged showing SRT1720 to have a thousand times more activation effect on SIRT1 than resveratrol (Milne et al., 2007; Smith et al., 2009), it is still unknown whether SRT1720 affects in vitro mat- uration of oocytes and promotes oocyte quality.
Through our studies, we have investigated the function of mito- chondria, morphology, and distribution of spindles and oocyte chro- mosomes from aged mice. Based on these results, we decided to assess the effects of SRT1720 for in vitro matured oocytes, as well as examine spindle, chromosome, and mitochondria of aged mice. The aim is to provide a theoretical basis of SRT1720 activity to improve the maturation and quality of oocytes from aged women.

⦁ | MATERIALS AND METHODS

⦁ | Materials and reagents

SRT1720 was dissolved in dimethyl sulfoxide (DMSO) and applied in the final reagents at concentrations of 0, 0.05, 0.1, and 1.0 μM. A cul- ture for in vitro maturation was created using medium 199 (M199) sup- plemented with 10% human serum albumin (HSA), 50-IU/ml penicillin, 5-μg streptomycin, 0.29-mmol/L sodium pyruvate, 0.15-IU/ml human chorionic gonadotropin (hCG), 0.075-U/ml recombinant follicle- stimulating hormone, 10-ng/ml recombinant human epidermal growth factor (EGF), and 10-μg/ml estradiol-17β (E2). The reagents used for immunofluorescence staining were anti-α-tubulin antibody, mouse

monoclonal (mouse IgG1 isotype), FITC-AffiniPure Goat Anti-Mouse IgG, and MitoTracker Green FM. All chemicals and reagents were pur- chased from Sigma-Aldrich Chemical Company (St Louis, MI, USA).

⦁ | MII-stage oocyte collection

C57BL/6J mice came from the laboratory animal center of Shandong University (Jinan, Shandong Province, China). Mice were divided into two groups: young mice (4–8 weeks) and aged mice (48–52 weeks). Female mice were injected intraperitoneally with 10-IU pregnant mare serum gonadotropin. Forty-eight hours later, the mice were injected with 10-IU hCG (Sigma-Aldrich). Two hundred twenty-seven meta- phase II (MII)-stage oocytes of aged mice and 234 MII-stage oocytes of young mice were collected from oviducts and put into M199 14 h after the hCG injection. Cumulus cells were aspirated by means of gentle pipetting in 80-IU hyaluronidase. MII-stage oocytes were incu- bated in a humid environment containing 5% carbon dioxide.

⦁ | GV-stage oocyte collection and in vitro maturation

Aged female mice (48–52 weeks) were injected intraperitoneally with 10-IU pregnant mare serum gonadotropin. Forty-eight hours later, germinal vesicle (GV)-stage oocytes were retrieved by puncturing the ovary with sterile needles. The oocytes were washed three times with M199. Immature oocytes were denuded of cumulus cells prior to in vitro maturation, and cumulus cells were aspirated by pipette. A total of 2011 denuded oocytes were cultured for 16 h with DMSO supplemented with in vitro maturation media (control), at three differ- ent SRT1720 concentrations (0.05, 0.1, 1.0 μM). Oocytes remained in an incubator with a humid environment containing 5% carbon dioxide at 37◦C. Oocyte maturity (MII stage) was checked under an inverted microscope.

⦁ | In vitro fertilization and embryo development

Spermatozoa were obtained from seminiferous ducts of male mice (>8 weeks), washed with in vitro fertilization (IVF) media, and incu- bated for 2 h for capacitation. Spermatozoa were incubated at 2– 5 × 106/ml density at 37◦C with a humidified atmosphere containing 5% carbon dioxide. After the spermatozoa had been incubated, the MII-stage oocytes were moved into a sperm droplet. Fertilization was assessed after 5–6 h. Two-cell stage was checked after 24 h, and blas- tocysts were checked after 108 h.

⦁ | Immunofluorescence and confocal analysis

MII-stage oocytes were fixed in 2% paraformaldehyde for 10 min at 37◦C and permeabilized with 0.2% Triton X-100 for 45 min. Oocytes

were incubated with monoclonal anti-α-tubulin antibody (diluted 1/100 in phosphate-buffered saline) for 90 min and FITC-AffiniPure Goat Anti-Mouse IgG (diluted 1/300 in phosphate-buffered saline) for 60 min. To specifically assess mitochondria distribution in the MII oocytes, oocytes were incubated with 400-nmol/L MitoTracker Green FM (diluted with phosphate-buffered saline) for 30 min and fixed in 2% paraformaldehyde for 10 min. All steps were performed at 37◦C. Chromatin in MII-stage oocytes was stained with Hoechst (B-2261; Sigma-Aldrich) for 10 min. Oocytes were then examined under a LSM780 confocal scanning microscope (ZEISS, Oberkochen, Germany).

⦁ | Criteria of spindle organization and chromosome alignment

The following criteria were applied to categorize spindle organization and chromosome alignment. Normal spindles were considered to be symmetrically shaped and to have a prominent pole on each side. Abnormal spindles were considered to be nonspindle shaped or asym- metric, and the length of the spindles had to be shortened, with some dissolution or missing spindles. It was regarded as normal chromo- some alignment when all the chromosomes arranged linearly on the spindle equatorial plate. On the contrary, abnormal chromosomes arrangment was characterized by chromosomes separation from the equatorial plate, excessively loose chromosomes arrangment, aggluti- nated chromosomes, and disordered chromosomes clutter distribution between the spindle fibers.

⦁ | Gene expression analysis by RT-PCR

For MII-stage oocyte characterization, the whole RNA was isolated using the RNeasy Micro Kit (QIAGEN, Hilden, Germany) and sub- jected to complementary DNA synthesis using a QuantiTect Reverse Transcription kit (QIAGEN). Polymerase chain reaction (PCR) reactions were done in duplicate with FastStart Universal SYBR Green Master (Roche, Basel, Switzerland) on an iCycler iQ5 real-time polymerase chain reaction (RT-PCR) detection system (Bio-Rad, Hercules, CA, USA). Twenty-microliter PCR reaction volume contained 10-μl SYBR Green PCR Master Mix, 1-μl complementary DNA template, 3-μl primer mixture, and 6-μl water. The primer pairs for RT-PCR of SIRT1 were forward CTGTTGACCGATGGACTCCT and reverse GCCACAGCGTCATATCATCC. The fold change of SIRT1 mRNA was calculated in relation to the house-keeping gene β-ACTIN, forward primer: 50-GCCGTCTTCCCCTCCATCGTG-30; reverse primer: 50- GGAGCCACACGCAGCTCATTGTAGA-30. The experiment was repeated three times independently.

⦁ | STATISTICAL ANALYSIS

At least three replicates were assessed for each treatment of aged mice. The average of the values was presented as mean SEM. The

comparison of rates data among different groups was performed using chi-square test with SPSS software (IBM Corp.). A P value of <0.05 was considered statistically significant.

⦁ | RESULTS

⦁ | Spindle morphology and chromosome arrangement in oocytes of aged mice

For normal mature oocytes, spindles are well organized and chromo- somes are located at the metaphase plate. In our sample, a higher pro- portion of aberrant spindles in oocytes from aged mice were observed compared with spindles from young mice (68.89% 1.93% vs. 20.80% 1.55%; P < 0.01). The main spindle phenotypes observed were partial or totally collapsed, and in some oocytes, spin- dles were even missing. The proportion of oocytes from aged mice with abnormal chromosome alignment was higher than that of young mice (63.63% 2.79% vs. 30.13% 1.89%; P < 0.01). For oocytes with abnormal chromosome alignment, chromosomes appeared to be disordered between spindle microtubules and without an obvious equatorial plate formation (Figure 1a,b). Immunofluorescence intensity of mitochondria in oocytes of aged mice was weaker than that in young mice (35.61 5.72 vs. 50.42 7.84; P < 0.01) (Figure 1c).

⦁ | SRT1720 enhanced oocyte maturation, fertilization, and blastocyst formation of aged mice

The extrusion rate of the first polar body of oocytes was significantly higher in the 0.1-μM SRT1720 group compared with the control group (0.1-μM DMSO) after 16 h in vitro maturation of the GV-stage oocytes from aged mice (P < 0.01). The fertilization rate of two pronuclei and blastocyst formation rate were significantly improved when cultured in 0.1-μM SRT1720 (P < 0.01) (Tables 1 and 2).

⦁ | 0.1-μM SRT1720 improved oocyte quality in aged mice

The percentage of normal spindles and chromosomes were improved in oocytes cultured in the 0.1-μM SRT1720 group compared with the control group (0.1-μM DMSO) (P < 0.01). The intensity of immunofluorescence staining for the mitochondria was higher in the 0.1-μM SRT1720 group compared with the control group (0.1-μM DMSO) (56.84 6.65 vs. 38.33 5.86) (P < 0.01) (Figure 2a,b)

⦁ | 0.1-μM SRT1720 upregulated the mRNA level of SRIT1 in oocytes from aged mice

We evaluated gene expression levels of SRIT1 for in vitro matured MII-stage oocytes after treatment with four concentrations of

FI GU R E 1 Spindle morphology, chromosome alignment and mitochondria intensity in oocytes from young and aged mice.
⦁ Immunofluorescence images of spindle and chromosomes distribution in young and aged oocytes. Spindles were stained with α-tubulin antibody (green) and chromosomes with Hoechst 33342 (red). (A) Spindles, (B) chromosomes, and
(C) merge of spindle and chromosomes from the young group (chromosomes were located at metaphase plate) and (D) spindles,
(E) chromosomes, and (F) merge of spindle and chromosomes from aged group. Scale
bar = 25 μm. (b) The percentages of abnormal
spindles (*P < 0.01) and chromosomes (*P < 0.01) in young and aged groups.
(c) Immunofluorescence intensity of mitochondria in young group and aged group (*P < 0.01)

TABL E 1 Rates of germinal vesicle break down (GVBD), MII, and degeneration of oocytes from aged mice treated with SRT1720

Group (μM) Oocyte (n) GV (%) GVBD (%) MII (%) Degeneration (%)
Control 482 8.82 1.66 9.13 1.20 78.73 1.70 3.33 0.73
0.05SRT1720 508 6.76 1.08 7.19 1.15 81.97 1.00 4.08 0.68
0.1SRT1720 514 2.97 1.30 3.56 1.26 *
91.19 1.15 2.29 0.98
1.0SRT1720 507 5.78 2.05 5.13 0.57 75.92 1.20 12.23 1.42
Note: Oocytes from 0.1-μM SRT1720-treated group showed significantly higher maturation rate. Values in bold are statistically significant. Abbreviations: GV, germinal vesicle; MII, metaphase II.
*P < 0.01.

TABL E 2 Rates of fertilization and blastocyst formation in aged mice treated with 0.1-μM SRT1720

Group Oocytes (n) Fertilization (n and %) Blastocyst (n and %)
Control 202 130 (64.43 1.27) 69 (34.04 3.09)
0.1SRT1720 202 *
158 (77.71 3.33) #
96 (47.28 2.13)
Note: Fertilization and blastocyst formation were significantly improved after in vitro fertilization of oocytes from SRT1720-treated group. Values in bold are statistically significant.
*P < 0.01.
#P < 0.01.

SRT1720. The level of SRIT1 was upregulated in oocytes treated with 0.1-μM SRT1720 in aged mice (P < 0.05). Treatment with other con- centrations of SRT1720 had no obvious effect on gene expression levels of SRIT1 (Figure 2c).

⦁ | DISCUSSION

Previous evidence has shown that fertility declines with age for both infertile women and for those individuals undergoing artificial
reproduction (Ron-El et al., 2000). Fertility decline could be due to a decrease in oocyte quality and quantity. The deterioration of oocyte quality may lead to several structural changes and a decrease in func- tional status of mammalian oocytes as indicated by fertilization rate reduction, abnormal mitochondria structure, and oxidative damage in aged animals (Au et al., 2005; Pasquariello et al., 2019). Therefore, antioxidative compounds have been studied to examine their protec- tive effects during oocyte aging. To analyze the changes of mitochon- dria, spindles, and chromosomes of oocytes in aged mice, we needed to explore the causes of oocyte quality deterioration in aged

FIGU RE 2 Effect of SRT1720 supplementation during in vitro culture on oocyte quality. (a) The percentages of abnormal spindles (*P < 0.01) and chromosomes
(*P < 0.01) in control and SRT1720 groups. (b) Immunofluorescence intensity of mitochondria (*P < 0.01).
(c) Effects of different concentrations of SRT1720 (0.05, 0.1, and 1.0 μM) on SIRT1 expression in oocytes
(*P < 0.05)

individuals. In our study, we showed that supplementing oocytes with 0.1-μM SRT1720 in culture improved oocyte quality and the develop- mental rate of blastocysts of aged mice.
Spindles are dynamical organelles composed of a large number of microtubules, which direct the movement, distribution, and polar dis- charge of chromosomes. Mistakes in chromosome segregation or distribution may cause aneuploid embryo formation, which may lead to spontaneous abortion (Wang & Sun, 2006). Evidence has shown that regulatory spindle factors may change in elderly mammals, resulting in abnormal spindle and chromosome structure and function in oocytes (Pellestor et al., 2003). In our study, we found that spindles partially or completely disintegrated in aged oocytes, and some spin- dles were even missing. The rate for abnormal spindle structures in MII-stage oocytes was significantly higher in aged mice than in young mice. Our data also show that there was a significant difference in how many chromosomes were abnormally aligned in MII-stage oocytes of aged mice compared with the control group. Thus, our results add to the growing body of evidence documenting how the decline of oocyte quality, embryo formation, and pregnancy might be related to abnormal spindle or chromosome formation in elderly females who are in need of assisted reproductive technologies.
Mitochondria are the most abundant organelle in cytoplasm, pro- viding energy for oocyte maturation and for other life activities (Cotterill et al., 2013). Mitochondria directly affect the developmental potential of oocytes and are closely related to embryo quality (May- Panloup et al., 2016). It has also been found that the mitochondrial DNA copy number decreased in oocytes of aged cows (Iwata et al., 2011). In our study, we found that fluorescence intensity of MII-stage oocytes was weaker in oocytes from aged mice than that from young mice. Lower levels of mitochondria can directly affect the distribution and arrangement of spindles and chromosomes, which leads to an increase of aneuploidy in oocytes.
Evidence has shown how SRT1720, a new chemical entity drug, can have strong antiaging and antiapoptosis effects in vitro (Li et al., 2016) and how it can improve mitochondria respiratory chain function (Funk et al., 2010; Milne et al., 2007; Smith et al., 2009). Researchers found that SIRT1 activation by resveratrol could promote oocyte developmental ability by improving mitochondrial function through enhancing mitochondria biosynthesis and degradation gene- sis (Sato et al., 2014). In our study, we estimated the effects of SRT1720 at three different concentrations of in vitro maturation media on oocytes from aged mice. The intensity of immunofluorescence staining of mitochondria was higher in the 0.1-μM SRT1720-treated group compared with the control group, indicating that SRT1720 could improve oocyte quality by enhancing mitochondrial function. Meanwhile, we also found that normal spindle morphology and chromosome alignment were significantly enhanced in MII-stage oocytes in the 0.1-μM SRT1720-treated group compared with the control group. As such, we postulated that SRT1720 could improve oocyte quality by enhancing spindle morphology and chro- mosome alignment in aged mice.
Our study further directed towards the regulative effects of SRT1720 on oocytes. We found that SRT1720 treatment significantly improved oocyte maturation for oocytes from aged mice. SRT1720 significantly enhanced oocyte fertilization, as well as blastocyst forma- tion. These results provided evidence for SRT1720’s role in improving oocyte development in aged mice. We assessed the effects of three different concentrations of SRT1720 in vitro maturation media on oocytes from aged mice and showed that 0.1-μM SRT1720 supple- mentation was the optimal concentration to achieve maturation.
It is well known that SIRT1 is a protective factor against oxidative stress in oocytes from mice (Donato et al., 2011; Jian et al., 2012; Lafontaine-Lacasse et al., 2010). Resveratrol and melatonin could increase the quality of aged MII-stage oocytes through the

upregulation of SIRT1 (Almohammed et al., 2020; Liu et al., 2018). SRT1720’s affinity with SIRT1 is about a thousand times stronger than resveratrol’s affinity to SIRT1 (Funk et al., 2010; Milne et al., 2007; Smith et al., 2009). Some authors showed that the gene expression of SIRT1 was modulated by the oxidative stress (Prozorovski et al., 2008). Yang et al. (2007) showed that the elevate ROS will decrease the SIRT1 expression. The results indicated that the SIRT1 may be an important target for preventing oocyte quality. Yang et al. (2018) reported that the SIRT1 activator SRT1720 could delay the oocyte aging by the reduced ROS content and meiotic in oocytes than that of aged oocytes. In our study, we found that the gene expression level of SIRT1 was higher in the 0.1-μM SRT1720 group when compared with the gene expression level found in the control group. The activation of SIRT1 expression is related to the reduced ROS level, antiapoptotic effects in mammals (Yang et al., 2015; Zhao et al., 2015). Thus, we speculated that SRT1720 could upregulate SIRT1 expression, enhance mitochondrial function, and inhibit meiosis defects, resulting in improved oocyte quality, oocyte maturation and embryonic development in aged mice.
In conclusion, our study shows that 0.1-μM SRT1720 supplement in vitro can improve oocytes maturation, quality, fertilization, and blastocyst formation from aged mice by improving mitochondrial function, spindle morphology, chromosome alignment, and SIRT1 upregulation. Our data have important implications in the field of assisted reproduction for elderly women. Further investigation is warranted to explore the underlying mechanisms of SRT1720 in aged mice and to investigate whether SRT1720 has any protective effects on oocytes from aged women.

ACKNOWLEDGMENTS
This work was supported by Shandong Provincial Medicine and Health Technology Development Project (2018WS338), the Joint Research Funding of Shandong University and Karolinska Institute (SDU-KI-2019-08), and Medical Doctor Innovation Fund of Linyi Peo- ple’s Hospital (2019LYBS07).

CONFLICT OF INTEREST
The authors declare no conflicts of interest.

ORCID
Mei-Ju Liu https://orcid.org/0000-0003-2717-6738

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